|Statement||editors, Christopher J. Lamb, Roger N. Beachy.|
|Series||UCLA symposia on molecular and cellular biology ;, new series, v. 129, UCLA symposia on molecular and cellular biology ;, new ser., v. 7.|
|Contributions||Lamb, Christopher J., Beachy, Roger N., UCLA Symposium on Plant Gene Transfer (1989 : Park City, Utah)|
|LC Classifications||QK981.5 .P568 1990|
|The Physical Object|
|Pagination||xx, 345 p. :|
|Number of Pages||345|
|LC Control Number||89070553|
Second, gene transfer technology has facilitated the molecular cloning, by tagging genomic sequences, of important genes (e. g., homeotic genes) whose gene products control the normal pattern of growth and differentia tion of plants. For A. tumefaciens-based gene transfer, the infection of a plant cell by the bacterium is a prerequisite. This occurs easily in nature only with dicots, while monocots are no host. Thus, this transfer technique is hampered by host-range limitations, which are, especially disturbing when aiming at . Direct gene transfer into plant cells or protoplasts by the use of microinjection, electroporation or biolistic systems, or mediated by Agrobacteria is described in detail for various plant species, including relevant crops and cereals. Horizontal gene transfer also plays a role in the spread of virulence factors, such as exotoxins and exoenzymes, amongst bacteria. A prime example concerning the spread of exotoxins is the adaptive evolution of Shiga toxins in E. coli through horizontal gene .
Nov 22, · GENE TRANSFER IN PLANTS Gene transfer or uptake of DNA refers to the process that moves a specific piece of DNA into cell. The directed desirable gene transfer from one organism to another and the subsequent stable integration & expression of foreign gene into the genome is referred as genetic transformation. plant science, a great progress in understanding the Agrobacterium-mediated gene transfer to plant cells has been archived. However, Agrobacterium tumefaciens naturally infects only dicotyledonous plants and many economically important plants, including the cereals, remained accessible for genetic manipulation during long time. Nov 08, · GENE TRANSFER in PLANT - authorSTREAM Presentation. Electroporation: Electroporation It involves a pulse of high voltage applied to protoplasts/cells/ tissues to make pores in the plasma membrane which facilitates the uptake of foreign DNA. from book Alien gene transfer in of selection of better plant types. Vertical and horizontal transfer of alien genes has changed the fateof several crops by imparting resistance to diseases.
Gene Transfer: Method # Virus Mediated Gene Transfer: In other way the gene can be packed into a virus and allow it to infect the host cell without harming it in any way. This method can be used both for the transformation of prokaryotic host cell as well as transfection of eukaryotic host cells. Genome sequencing of several organisms has resulted in the rapid progress of genomic studies. Genetic transformation is a powerful tool and an important technique for the study of plant functional genomics, i.e., gene discovery, new insights into gene function, and investigation of Cited by: Methods of Gene Transfer in Plants. To add a desired trait to a crop, a foreign gene (transgene) encoding the trait must be inserted into plant cells, along with a “cassette” of additional genetic material. Volumes 1 and 2 of Transgenic Plants assemble important information on transgenic crops which has appeared scattered in many different publications. These two volumes are a significant milestone in plant/agricultural biology, promote the practical application of recombinant DNA technology, and assist in transforming the agricultural industry.